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il17a  (R&D Systems)


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    Structured Review

    R&D Systems il17a
    Il17a, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+il+17a/Recombinant+Mouse+IL-17A+Protein/pm41663713-60-12-18
    Average 95 stars, based on 100 article reviews
    il17a - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Herpesvirus-Entry Mediator Inhibits the NF- κ B Pathway Activated by IL-17 and Fosters the Osteogenic Differentiation of Allogeneic Mesenchymal Stem Cells
    Article Snippet: .. These cells were cultured in the osteogenic differentiation medium (OriCell C57BL/6 MSC Osteogenic Differentiation Medium, Cyagen Biosciences, China) in the presence or absence of 50 ng/mL recombinant mouse IL-17A (#7956-ML, R&D Systems, USA). ..

    Article Title: Candida albicans Promotes Oral Cancer via IL-17A/IL-17RA-Macrophage Axis.
    Article Snippet: Transwell chamber (8.0-mm pore membranes; Corning) was used to explore the migration of macrophages. .. SCC VII cells (1 104 cells per well) were seeded in the lower chamber and incubated for 24 h. Then, the culture medium was replaced with 600 mL serum-free medium supplemented with 0, 50, or 100 ng/mL recombinant mouse IL-17A (rmIL-17A, 421-ML, R&D Systems) and cultured for 8 h. After that, 200 mL RAW 264.7 cells (5 105/mL) in serum-free medium were added into the upper chamber. ..

    Article Title: Candida albicans Promotes Oral Cancer via IL-17A/IL-17RA-Macrophage Axis
    Article Snippet: Transwell chamber (8.0-μm pore membranes; Corning) was used to explore the migration of macrophages. .. SCC VII cells (1 × 10 4 cells per well) were seeded in the lower chamber and incubated for 24 h. Then, the culture medium was replaced with 600 μL serum-free medium supplemented with 0, 50, or 100 ng/mL recombinant mouse IL-17A (rmIL-17A, 421-ML, R&D Systems) and cultured for 8 h. After that, 200 μL RAW 264.7 cells (5 × 10 5 /mL) in serum-free medium were added into the upper chamber. ..

    Recombinant:

    Article Title: Herpesvirus-Entry Mediator Inhibits the NF- κ B Pathway Activated by IL-17 and Fosters the Osteogenic Differentiation of Allogeneic Mesenchymal Stem Cells
    Article Snippet: .. These cells were cultured in the osteogenic differentiation medium (OriCell C57BL/6 MSC Osteogenic Differentiation Medium, Cyagen Biosciences, China) in the presence or absence of 50 ng/mL recombinant mouse IL-17A (#7956-ML, R&D Systems, USA). ..

    Article Title: Candida albicans Promotes Oral Cancer via IL-17A/IL-17RA-Macrophage Axis.
    Article Snippet: Transwell chamber (8.0-mm pore membranes; Corning) was used to explore the migration of macrophages. .. SCC VII cells (1 104 cells per well) were seeded in the lower chamber and incubated for 24 h. Then, the culture medium was replaced with 600 mL serum-free medium supplemented with 0, 50, or 100 ng/mL recombinant mouse IL-17A (rmIL-17A, 421-ML, R&D Systems) and cultured for 8 h. After that, 200 mL RAW 264.7 cells (5 105/mL) in serum-free medium were added into the upper chamber. ..

    Article Title: Immune control of the basal ganglia network: Interleukin-17 as a key modulator of striatal synaptic plasticity.
    Article Snippet: Striatal IL-17A levels were assessed through ELISA assays (Duoset R&D system, Assay Range: 16-1000 pg/ml). .. Recombinant mouse IL-17A was purchased from R&D Systems (Minneapolis, U.S.A.); ARA014418 and NVP were purchased from Sigma-Aldrich (Milan, Italy), Ifenprodil and Picrotoxin were purchased from Tocris bioscience (Bristol, UK). ..

    Article Title: Interleukin-17 receptor C is essential for the pro-inflammatory pathogenicity of granulocyte-macrophage-colony-stimulating factor-producing T helper cells in experimental autoimmune uveitis.
    Article Snippet: Autoimmune uveitis is an inflammatory disorder of the eye triggered by the responses of autoreactive T cells to ocular autoantigens.. This study aims to understand the role of granulocyte–macrophage-colony-stimulating factor (GM-CSF)-producing T helper (ThGM) cells in the pathophysiology of mouse experimental autoimmune uveitis (EAU).. We established an EAU model by immunizing mice with interphotoreceptor retinoid-binding protein (IRBP) 651–670.

    Article Title: Role of ADAM10/17-Mediated Cleavage of LAG3 in the Impairment of Immunosuppression in Psoriasis.
    Article Snippet: Despite extensive research on immune activation regulatory mechanisms, studies on immune suppression in psoriasis are limited.. LAG3, a newly identified immune checkpoint, plays a crucial role in modulating immune responses and maintaining T-regulatory cell function.. However, its involvement in psoriasis is unclear.

    Article Title: Ropinirole inhibits inflammatory cytokine production in gingival epithelial cells and suppresses alveolar bone loss in an experimental rat model of periodontitis
    Article Snippet: .. We used l-carrageenan (carrageenan; WAKO Pure Chemical Industries Ltd., Osaka, Japan), ropinirole (Sigma-Aldrich Japan, Tokyo, Japan) as a D2-like receptor agonist, haloperidol (WAKO Pure Chemical Industries Ltd.) as D2-like receptor antagonist, and carrier-free recombinant mouse IL-17A (rmIL-17A; R&D Systems, Guthrie, MN, USA). ..

    Article Title: Candida albicans Promotes Oral Cancer via IL-17A/IL-17RA-Macrophage Axis
    Article Snippet: Transwell chamber (8.0-μm pore membranes; Corning) was used to explore the migration of macrophages. .. SCC VII cells (1 × 10 4 cells per well) were seeded in the lower chamber and incubated for 24 h. Then, the culture medium was replaced with 600 μL serum-free medium supplemented with 0, 50, or 100 ng/mL recombinant mouse IL-17A (rmIL-17A, 421-ML, R&D Systems) and cultured for 8 h. After that, 200 μL RAW 264.7 cells (5 × 10 5 /mL) in serum-free medium were added into the upper chamber. ..

    Article Title: An IL-17-DUOX2 axis controls gastrointestinal colonization by Candida albicans
    Article Snippet: Isolated colonic epithelial cells were mixed with ice-cold Cultrex reduced growth factor basement membrane type R1 (R&D Systems) and cultured for 5 days in 50% conditioned medium containing wnt3a, R-spondin-3, noggin, and 20% fetal bovine serum supplemented with Chir99021 (5 μM), Thiazovivin (2.5 μM), and Primocin (100 μg/mL). .. On day 4, colonoids were challenged with sonicates of C. albicans SC5314 in yeast and hyphal forms (10 7 cells/mL); β-1,3-curdlan from Alcaligenes faecalis (100 μg/mL in DMSO; Invivogen); mannan (250 μg/mL in 1:1 PBS/DMSO solution; Millipore-Sigma), zymosan A (250 μg/mL in 1:1 PBS/DMSO solution; Millipore-Sigma), β-glucan from Saccharomyces cerevisiae (100 μg/mL in 1:1 PBS/DMSO solution; Millipore-Sigma); recombinant mouse IL-17A (5 ng/mL; R&D Systems); or the appropriate vehicles and carrier proteins for 24 h. All ligands and cytokines were preincubated with polymyxin B (PMB; 25 μg/mL; Millipore-Sigma) for 30 min at 37°C to prevent activation by lipopolysaccharide (LPS) contamination. .. Ultrapure LPS (1 μg/mL; Invivogen) was used as a positive control for the induction of Duox2 .

    Incubation:

    Article Title: Candida albicans Promotes Oral Cancer via IL-17A/IL-17RA-Macrophage Axis.
    Article Snippet: Transwell chamber (8.0-mm pore membranes; Corning) was used to explore the migration of macrophages. .. SCC VII cells (1 104 cells per well) were seeded in the lower chamber and incubated for 24 h. Then, the culture medium was replaced with 600 mL serum-free medium supplemented with 0, 50, or 100 ng/mL recombinant mouse IL-17A (rmIL-17A, 421-ML, R&D Systems) and cultured for 8 h. After that, 200 mL RAW 264.7 cells (5 105/mL) in serum-free medium were added into the upper chamber. ..

    Article Title: Interleukin-17 receptor C is essential for the pro-inflammatory pathogenicity of granulocyte-macrophage-colony-stimulating factor-producing T helper cells in experimental autoimmune uveitis.
    Article Snippet: Autoimmune uveitis is an inflammatory disorder of the eye triggered by the responses of autoreactive T cells to ocular autoantigens.. This study aims to understand the role of granulocyte–macrophage-colony-stimulating factor (GM-CSF)-producing T helper (ThGM) cells in the pathophysiology of mouse experimental autoimmune uveitis (EAU).. We established an EAU model by immunizing mice with interphotoreceptor retinoid-binding protein (IRBP) 651–670.

    Article Title: Candida albicans Promotes Oral Cancer via IL-17A/IL-17RA-Macrophage Axis
    Article Snippet: Transwell chamber (8.0-μm pore membranes; Corning) was used to explore the migration of macrophages. .. SCC VII cells (1 × 10 4 cells per well) were seeded in the lower chamber and incubated for 24 h. Then, the culture medium was replaced with 600 μL serum-free medium supplemented with 0, 50, or 100 ng/mL recombinant mouse IL-17A (rmIL-17A, 421-ML, R&D Systems) and cultured for 8 h. After that, 200 μL RAW 264.7 cells (5 × 10 5 /mL) in serum-free medium were added into the upper chamber. ..

    Injection:

    Article Title: Role of ADAM10/17-Mediated Cleavage of LAG3 in the Impairment of Immunosuppression in Psoriasis.
    Article Snippet: Despite extensive research on immune activation regulatory mechanisms, studies on immune suppression in psoriasis are limited.. LAG3, a newly identified immune checkpoint, plays a crucial role in modulating immune responses and maintaining T-regulatory cell function.. However, its involvement in psoriasis is unclear.

    Activation Assay:

    Article Title: An IL-17-DUOX2 axis controls gastrointestinal colonization by Candida albicans
    Article Snippet: Isolated colonic epithelial cells were mixed with ice-cold Cultrex reduced growth factor basement membrane type R1 (R&D Systems) and cultured for 5 days in 50% conditioned medium containing wnt3a, R-spondin-3, noggin, and 20% fetal bovine serum supplemented with Chir99021 (5 μM), Thiazovivin (2.5 μM), and Primocin (100 μg/mL). .. On day 4, colonoids were challenged with sonicates of C. albicans SC5314 in yeast and hyphal forms (10 7 cells/mL); β-1,3-curdlan from Alcaligenes faecalis (100 μg/mL in DMSO; Invivogen); mannan (250 μg/mL in 1:1 PBS/DMSO solution; Millipore-Sigma), zymosan A (250 μg/mL in 1:1 PBS/DMSO solution; Millipore-Sigma), β-glucan from Saccharomyces cerevisiae (100 μg/mL in 1:1 PBS/DMSO solution; Millipore-Sigma); recombinant mouse IL-17A (5 ng/mL; R&D Systems); or the appropriate vehicles and carrier proteins for 24 h. All ligands and cytokines were preincubated with polymyxin B (PMB; 25 μg/mL; Millipore-Sigma) for 30 min at 37°C to prevent activation by lipopolysaccharide (LPS) contamination. .. Ultrapure LPS (1 μg/mL; Invivogen) was used as a positive control for the induction of Duox2 .



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    a–c, Proposed model illustrating how effector cytokines produced by infiltrating Th1 and Th17 lymphocytes following repeated GAS infections may differentially shape transcriptional responses in brain endothelial cells (BECs) and microglia. a, IFNγ is predicted to contribute to the induction of interferon-response programs and antigen-presentation pathways in both microglia and BECs. <t>b,c,</t> <t>IL-17A</t> and GM-CSF appear to exert partially overlapping effects on microglial activation, including regulation of proliferative responses and induction of disease-associated microglial (DAM) genes, cytokines, and chemokines, while also displaying cytokine-specific contributions. Notably, IL-17A signaling may more strongly influence endothelial transcriptional alterations following GAS infections. In addition, antigen-presentation signatures remain elevated in conditions of Th17 deficiency or systemic IL-17A neutralization, suggesting that IL-17A may normally modulate these pathways in both microglia and BECs. Together, these models highlight shared and distinct roles for Th17-associated cytokines in shaping neuroimmune and vascular responses after recurrent GAS exposure.
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    a–c, Proposed model illustrating how effector cytokines produced by infiltrating Th1 and Th17 lymphocytes following repeated GAS infections may differentially shape transcriptional responses in brain endothelial cells (BECs) and microglia. a, IFNγ is predicted to contribute to the induction of interferon-response programs and antigen-presentation pathways in both microglia and BECs. <t>b,c,</t> <t>IL-17A</t> and GM-CSF appear to exert partially overlapping effects on microglial activation, including regulation of proliferative responses and induction of disease-associated microglial (DAM) genes, cytokines, and chemokines, while also displaying cytokine-specific contributions. Notably, IL-17A signaling may more strongly influence endothelial transcriptional alterations following GAS infections. In addition, antigen-presentation signatures remain elevated in conditions of Th17 deficiency or systemic IL-17A neutralization, suggesting that IL-17A may normally modulate these pathways in both microglia and BECs. Together, these models highlight shared and distinct roles for Th17-associated cytokines in shaping neuroimmune and vascular responses after recurrent GAS exposure.
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    Image Search Results


    a–c, Proposed model illustrating how effector cytokines produced by infiltrating Th1 and Th17 lymphocytes following repeated GAS infections may differentially shape transcriptional responses in brain endothelial cells (BECs) and microglia. a, IFNγ is predicted to contribute to the induction of interferon-response programs and antigen-presentation pathways in both microglia and BECs. b,c, IL-17A and GM-CSF appear to exert partially overlapping effects on microglial activation, including regulation of proliferative responses and induction of disease-associated microglial (DAM) genes, cytokines, and chemokines, while also displaying cytokine-specific contributions. Notably, IL-17A signaling may more strongly influence endothelial transcriptional alterations following GAS infections. In addition, antigen-presentation signatures remain elevated in conditions of Th17 deficiency or systemic IL-17A neutralization, suggesting that IL-17A may normally modulate these pathways in both microglia and BECs. Together, these models highlight shared and distinct roles for Th17-associated cytokines in shaping neuroimmune and vascular responses after recurrent GAS exposure.

    Journal: bioRxiv

    Article Title: Th17 effector cytokines induce shared and distinct microglial and endothelial cell responses in post-streptococcal encephalitis

    doi: 10.64898/2026.02.04.703836

    Figure Lengend Snippet: a–c, Proposed model illustrating how effector cytokines produced by infiltrating Th1 and Th17 lymphocytes following repeated GAS infections may differentially shape transcriptional responses in brain endothelial cells (BECs) and microglia. a, IFNγ is predicted to contribute to the induction of interferon-response programs and antigen-presentation pathways in both microglia and BECs. b,c, IL-17A and GM-CSF appear to exert partially overlapping effects on microglial activation, including regulation of proliferative responses and induction of disease-associated microglial (DAM) genes, cytokines, and chemokines, while also displaying cytokine-specific contributions. Notably, IL-17A signaling may more strongly influence endothelial transcriptional alterations following GAS infections. In addition, antigen-presentation signatures remain elevated in conditions of Th17 deficiency or systemic IL-17A neutralization, suggesting that IL-17A may normally modulate these pathways in both microglia and BECs. Together, these models highlight shared and distinct roles for Th17-associated cytokines in shaping neuroimmune and vascular responses after recurrent GAS exposure.

    Article Snippet: Media containing either mouse IFNγ (R&D Systems, 485-MI-100), mouse IL-17A (R&D Systems, 7956-ML-025), mouse GM-CSF (R&D Systems, 415-ML-010) or vehicle (PBS with Ca 2+ and Mg 2+ ) was applied to corresponding wells.

    Techniques: Produced, Immunopeptidomics, Activation Assay, Neutralization

    a, Timeline of recurrent intranasal GAS infections and administration of an α–IL-17A–neutralizing monoclonal antibody (mAb) or isotype control. b, Heat maps of differentially expressed genes (DEGs) related to BBB function, LPS response, interferon signaling, and antigen presentation in olfactory bulb (OB) brain endothelial cells (BECs) from GAS-infected mice treated with isotype control or α–IL-17A mAb. Values are shown as log(z-score); significantly altered genes are indicated in black (adjusted p < 0.05). c, Gene ontology (GO) pathway enrichment analysis of transcripts upregulated and downregulated in α–IL-17A mAb–treated versus isotype-treated BECs following GAS infections. d, Representative immunofluorescence (IF) images of endogenous IgG leakage (green) in the granular layer of the OB. Blood vessels are labeled with Glut1 (cyan). e, f, Quantification of IgG extravasation (relative fluorescence intensity) in the glomerular ( e ) and granular ( f ) OB layers from PBS- or GAS-infected mice treated with α–IL-17A mAb or isotype control. Comparisons were performed using two-way ANOVA with Šídák’s multiple comparisons test ( p < 0.05; ** p < 0.001; n = 3–7 mice per group). g–i, Representative IF images for IFITM3 (pink), Iba1 (yellow), and CD31 (blue) in the OB of GAS-infected mice treated with isotype or α–IL-17A mAb ( g,h ), and quantification of Ifitm3-positive area within CD31⁺ vasculature ( i ). j,k, Representative fluorescence in situ hybridization (FISH) images of Itm2a mRNA (pink) combined with Glut1 (blue) in the glomerular layer ( j ) and quantification of vascular Itm2a signal ( k ). l, Representative IF images of the tight junction proteins Claudin-5 (green) and ZO-1 (red) in the OB vasculature of GAS-infected mice treated with isotype or α–IL-17A mAb. m, Serum cytokine concentrations measured by multiplex immunoassay in PBS- or GAS-infected mice treated with isotype or α–IL-17A mAb. Data are mean ± SEM. Comparisons were performed using one-way ANOVA with Tukey’s multiple comparisons test (ns, p > 0.05; p < 0.05; * p < 0.01; ** p < 0.001; *** p < 0.0001; n = 3 - 6 mice per group). n, Survival curves of GAS-infected mice treated with isotype control or α–IL-17A mAb (n = 13–48 mice per group). Statistical significance was assessed using the Mantel–Cox log-rank test.

    Journal: bioRxiv

    Article Title: Th17 effector cytokines induce shared and distinct microglial and endothelial cell responses in post-streptococcal encephalitis

    doi: 10.64898/2026.02.04.703836

    Figure Lengend Snippet: a, Timeline of recurrent intranasal GAS infections and administration of an α–IL-17A–neutralizing monoclonal antibody (mAb) or isotype control. b, Heat maps of differentially expressed genes (DEGs) related to BBB function, LPS response, interferon signaling, and antigen presentation in olfactory bulb (OB) brain endothelial cells (BECs) from GAS-infected mice treated with isotype control or α–IL-17A mAb. Values are shown as log(z-score); significantly altered genes are indicated in black (adjusted p < 0.05). c, Gene ontology (GO) pathway enrichment analysis of transcripts upregulated and downregulated in α–IL-17A mAb–treated versus isotype-treated BECs following GAS infections. d, Representative immunofluorescence (IF) images of endogenous IgG leakage (green) in the granular layer of the OB. Blood vessels are labeled with Glut1 (cyan). e, f, Quantification of IgG extravasation (relative fluorescence intensity) in the glomerular ( e ) and granular ( f ) OB layers from PBS- or GAS-infected mice treated with α–IL-17A mAb or isotype control. Comparisons were performed using two-way ANOVA with Šídák’s multiple comparisons test ( p < 0.05; ** p < 0.001; n = 3–7 mice per group). g–i, Representative IF images for IFITM3 (pink), Iba1 (yellow), and CD31 (blue) in the OB of GAS-infected mice treated with isotype or α–IL-17A mAb ( g,h ), and quantification of Ifitm3-positive area within CD31⁺ vasculature ( i ). j,k, Representative fluorescence in situ hybridization (FISH) images of Itm2a mRNA (pink) combined with Glut1 (blue) in the glomerular layer ( j ) and quantification of vascular Itm2a signal ( k ). l, Representative IF images of the tight junction proteins Claudin-5 (green) and ZO-1 (red) in the OB vasculature of GAS-infected mice treated with isotype or α–IL-17A mAb. m, Serum cytokine concentrations measured by multiplex immunoassay in PBS- or GAS-infected mice treated with isotype or α–IL-17A mAb. Data are mean ± SEM. Comparisons were performed using one-way ANOVA with Tukey’s multiple comparisons test (ns, p > 0.05; p < 0.05; * p < 0.01; ** p < 0.001; *** p < 0.0001; n = 3 - 6 mice per group). n, Survival curves of GAS-infected mice treated with isotype control or α–IL-17A mAb (n = 13–48 mice per group). Statistical significance was assessed using the Mantel–Cox log-rank test.

    Article Snippet: Media containing either mouse IFNγ (R&D Systems, 485-MI-100), mouse IL-17A (R&D Systems, 7956-ML-025), mouse GM-CSF (R&D Systems, 415-ML-010) or vehicle (PBS with Ca 2+ and Mg 2+ ) was applied to corresponding wells.

    Techniques: Control, Immunopeptidomics, Olfactory, Infection, Immunofluorescence, Labeling, Fluorescence, In Situ Hybridization, Multiplex Assay